• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

GMPR 1/2 Polyclonal antibody

GMPR 1/2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15683-1-AP

Synonyms

GMP reductase 1, GMPR, GMPR1, GMPR2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse skin tissue using GMPR 1/2 Polyclonal antibody (15683-1-AP)

    WB analysis of mouse skin using 15683-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 15683-1-AP (GMPR 1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using GMPR 1/2 Polyclonal antibody (15683-1-AP)

    WB analysis of mouse skeletal muscle using 15683-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15683-1-AP (GMPR 1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GMPR 1/2 Polyclonal antibody (15683-1-AP)

    IHC staining of human colon cancer using 15683-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15683-1-AP (GMPR 1/2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GMPR 1/2 Polyclonal antibody (15683-1-AP)

    IHC staining of human colon cancer using 15683-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15683-1-AP (GMPR 1/2 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse skin tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15683-1-AP targets GMPR 1/2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GMPR 1/2 fusion protein Ag8185
Full Name guanosine monophosphate reductase
Calculated Molecular Weight 345 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC008281
Gene Symbol GMPR
Gene ID (NCBI) 2766
RRIDAB_2111072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMPR (Guanosine monophosphate reductase) catalyzes the irreversible NADPH-dependent reductive deamination of guanosine monophosphate (GMP) to inosine monophosphate (IMP). GMPR is able to convert guanosine nucleotides to the pivotal precursor of both guanine (G) and adenine (A) nucleotides. It plays an important role in maintaining the intracellular balance of A and G nucleotides. This antibody can recognize both GMPR1 and GMPR2 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for GMPR 1/2 antibody 15683-1-APDownload protocol
IHC protocol for GMPR 1/2 antibody 15683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Regulation of local GTP availability controls RAC1 activity and cell invasion

Authors - Anna Bianchi-Smiraglia
View Article
humanWB,IF,IP

Cell Chem Biol

Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4.

Authors - David W Wolff
View Article
mouseWB

Cancer Prev Res (Phila)

Reducing Fatty Acid Oxidation Improves Cancer-free Survival in a Mouse Model of Li-Fraumeni Syndrome.

Authors - Ping-Yuan Wang
View Article
mouseWB

Cell Rep

Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.

Authors - Nicole M Sayles
View Article