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GNB4 Polyclonal antibody

GNB4 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 11978-2-AP

Synonyms

GNB4, Transducin beta chain 4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GNB4 Polyclonal antibody (11978-2-AP)

    WB analysis using 11978-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11978-2-AP (GNB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GNB4 Polyclonal antibody (11978-2-AP)

    IP experiment of mouse brain using 11978-2-AP

    IP result of anti-GNB4 (IP:11978-2-AP, 4ug; Detection:11978-2-AP 1:800) with mouse brain tissue lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using GNB4 Polyclonal antibody (11978-2-AP)

    IF Staining of SH-SY5Y using 11978-2-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 11978-2-AP (GNB4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HEK-293T cells, MCF-7 cells, SH-SY5Y cells, human placenta tissue, mouse kidney tissue
Positive IP detected inmouse brain tissue
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11978-2-AP targets GNB4 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNB4 fusion protein Ag2604
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 4
Calculated Molecular Weight 340 aa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC000873
Gene Symbol GNB4
Gene ID (NCBI) 59345
RRIDAB_2109620
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

G protein subunit beta 4 (GNB4), is one of the heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins. GNB4 is important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

Protocols

Product Specific Protocols
WB protocol for GNB4 antibody 11978-2-APDownload protocol
IF protocol for GNB4 antibody 11978-2-APDownload protocol
IP protocol for GNB4 antibody 11978-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

BMC Med

Helicobacter pylori-induced aberrant demethylation and expression of GNB4 promotes gastric carcinogenesis via the Hippo-YAP1 pathway

Authors - Duanrui Liu
View Article
humanWB

Nat Commun

Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo

Authors - Jasjot Singh
View Article