GPAM Polyclonal antibody
GPAM Polyclonal Antibody for IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse
Applications
IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12454-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12454-1-AP targets GPAM in IP, IHC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GPAM fusion protein Ag3120 |
| Full Name | glycerol-3-phosphate acyltransferase, mitochondrial |
| Calculated Molecular Weight | 828 aa, 94 kDa |
| Observed Molecular Weight | 90-92 kDa |
| GenBank Accession Number | BC030783 |
| Gene Symbol | GPAM |
| Gene ID (NCBI) | 57678 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GPAM (Glycerol-3-Phosphate Acyltransferase, Mitochondrial) is a Protein Coding gene. This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene.Diseases associated with GPAM include Rhizomelic Chondrodysplasia Punctata, Type 2 and Congenital Generalized Lipodystrophy. GPAM can be detected as 90-92 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GPAM antibody 12454-1-AP | Download protocol |
| IP protocol for GPAM antibody 12454-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
