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GPR137B Polyclonal antibody

GPR137B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16496-1-AP

Synonyms

GPR137B, TM7SF1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human heart tissue using GPR137B Polyclonal antibody (16496-1-AP)

    WB analysis of human heart using 16496-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16496-1-AP (GPR137B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using GPR137B Polyclonal antibody (16496-1-AP)

    WB analysis of human heart using 16496-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16496-1-AP (GPR137B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GPR137B Polyclonal antibody (16496-1-AP)

    IHC staining of human liver cancer using 16496-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16496-1-AP (GPR137B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using GPR137B Polyclonal antibody (16496-1-AP)

    IHC staining of human kidney using 16496-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16496-1-AP (GPR137B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using GPR137B Polyclonal antibody (16496-1-AP)

    IHC staining of human kidney using 16496-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16496-1-AP (GPR137B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GPR137B Polyclonal antibody (16496-1-AP)

    IHC staining of human liver cancer using 16496-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16496-1-AP (GPR137B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:100-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16496-1-AP targets GPR137B in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR137B fusion protein Ag9633
Full Name G protein-coupled receptor 137B
Calculated Molecular Weight 399 aa, 46 kDa
Observed Molecular Weight 48-50 kDa
GenBank Accession NumberBC012100
Gene Symbol GPR137B
Gene ID (NCBI) 7107
RRIDAB_2247856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GPR137B antibody 16496-1-APDownload protocol
IHC protocol for GPR137B antibody 16496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Bone

A role for G protein-coupled receptor 137b in bone remodeling in mouse and zebrafish.

Authors - K Urso
View Article
mouseWB

Biochem Biophys Res Commun

TM7SF1, an important autophagy regulatory protein in mouse podocytes.

Authors - Yunfei Hu
View Article