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  • Featured Product
  • KD/KO Validated

GPR35 Polyclonal antibody

GPR35 Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 55248-1-AP

Synonyms

G protein coupled receptor 35, GPR35, KYNA receptor, Kynurenic acid receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using GPR35 Polyclonal antibody (55248-1-AP)

    WB analysis of A549 using 55248-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using GPR35 Polyclonal antibody (55248-1-AP)

    WB analysis of human brain using 55248-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human stomach tissue using GPR35 Polyclonal antibody (55248-1-AP)

    WB analysis of human stomach using 55248-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using GPR35 Polyclonal antibody (55248-1-AP)

    WB analysis of A549 using 55248-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 55248-1-AP (GPR35 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using GPR35 Polyclonal antibody (55248-1-AP)

    FC experiment of A549 using 55248-1-AP

    1X10^6 A549 cells were stained with 0.2ug GPR35 antibody (55248-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inA549 cells, human brain tissue, human stomach tissue
Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55248-1-AP targets GPR35 in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name G protein-coupled receptor 35
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberNM_005301
Gene Symbol GPR35
Gene ID (NCBI) 2859
RRIDAB_10836935
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR35, also named as KYNA receptor, belongs to the G-protein coupled receptor 1 family. GPR35 acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of GPR35 is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins (PMID:16754668). This antibody is specific to GPR35.

Protocols

Product Specific Protocols
WB protocol for GPR35 antibody 55248-1-APDownload protocol
FC protocol for GPR35 antibody 55248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Acta Pharm Sin B

Functional metabolomics reveal the role of AHR/GPR35 mediated kynurenic acid gradient sensing in chemotherapy-induced intestinal damage.

Authors - Di Wang
  • KD Validated
View Article
humanWB

Eur J Pharmacol

Aminosalicylates target GPR35, partly contributing to the prevention of DSS-induced colitis

Authors - Wuxiyar Otkur
View Article