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GRIPAP1 Polyclonal antibody

GRIPAP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15806-1-AP

Synonyms

GRASP 1, GRIP1 associated protein 1, GRIPAP1, KIAA1167

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GRIPAP1 Polyclonal antibody (15806-1-AP)

    WB analysis using 15806-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15806-1-AP (GRIPAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GRIPAP1 Polyclonal antibody (15806-1-AP)

    IHC staining of mouse brain using 15806-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15806-1-AP (GRIPAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GRIPAP1 Polyclonal antibody (15806-1-AP)

    IHC staining of mouse brain using 15806-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15806-1-AP (GRIPAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using GRIPAP1 Polyclonal antibody (15806-1-AP)

    IF Staining of SH-SY5Y using 15806-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using GRIPAP1 antibody (15806-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15806-1-AP targets GRIPAP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRIPAP1 fusion protein Ag8540
Full Name GRIP1 associated protein 1
Calculated Molecular Weight 841 aa, 96 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC001522
Gene Symbol GRIPAP1
Gene ID (NCBI) 56850
RRIDAB_2112787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRIP-associated protein-1 (GRASP-1), also named as GRIPAP1, is a neuron-specific effector of the small GTPase Rab4 and key component of AMPA receptor recycling machinery in dendrites. GRASP-1 is essential for maintenance of spine morphology and important for LTP. GRASP-1 connects Rab4 and Rab11 recycling endosomal domains through the interaction with target (t)-SNARE syntaxin. The MW of this protein is 97 kDa, and Catalog#15806-1-AP specially recognises the 97 kDa protein.

Protocols

Product Specific Protocols
WB protocol for GRIPAP1 antibody 15806-1-APDownload protocol
IHC protocol for GRIPAP1 antibody 15806-1-APDownload protocol
IF protocol for GRIPAP1 antibody 15806-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Modulated Expression of Specific tRNAs Drives Gene Expression and Cancer Progression.

Authors - Hani Goodarzi
View Article