• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

GSDMD Monoclonal antibody

GSDMD Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E6C7

Cat no : 66387-1-Ig

Synonyms

DF5L, DFNA5L, FKSG10, gasdermin D, GSDMD, GSDMDC1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GSDMD Monoclonal antibody (66387-1-Ig)

    WB analysis using 66387-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using GSDMD Monoclonal antibody (66387-1-Ig)

    WB analysis using 66387-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human small intestine tissue using GSDMD Monoclonal antibody (66387-1-Ig)

    WB analysis of human small intestine using 66387-1-Ig

    human small intestine tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using GSDMD Monoclonal antibody (66387-1-Ig)

    WB analysis of human colon using 66387-1-Ig

    human colon tissue were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using GSDMD Monoclonal antibody (66387-1-Ig)

    WB analysis of A431 using 66387-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of EC109 cells using GSDMD Monoclonal antibody (66387-1-Ig)

    WB analysis of EC109 using 66387-1-Ig

    EC109 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using GSDMD Monoclonal antibody (66387-1-Ig)

    WB analysis of COLO 320 using 66387-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using GSDMD Monoclonal antibody (66387-1-Ig)

    WB analysis of Raji using 66387-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using GSDMD Monoclonal antibody (66387-1-Ig)

    WB analysis of Raji using 66387-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using GSDMD Monoclonal antibody (66387-1-Ig)

    WB analysis of K-562 using 66387-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using GSDMD Monoclonal antibody (66387-1-Ig)

    WB analysis of HL-60 using 66387-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of TF-1 cells using GSDMD Monoclonal antibody (66387-1-Ig)

    WB analysis of TF-1 using 66387-1-Ig

    TF-1 cells were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GSDMD Monoclonal antibody (66387-1-Ig)

    IF Staining of HeLa using 66387-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66387-1-Ig(GSDMD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inU2OS cells, HEK-293 cells, A431 cells, EC109 cells, Raji cells, K-562 cells, COLO 320 cells, HL-60 cells, TF-1 cells, human small intestine tissue, human colon tissue, PC-3 cells, A549 cells, HepG2 cells, Jurkat cells, THP-1 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66387-1-Ig targets GSDMD in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GSDMD fusion protein Ag16827
Full Name gasdermin D
Calculated Molecular Weight 484 aa, 53 kDa
Observed Molecular Weight 47-53 kDa
GenBank Accession NumberBC008904
Gene Symbol GSDMD
Gene ID (NCBI) 79792
RRIDAB_2881763
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

Protocols

Product Specific Protocols
WB protocol for GSDMD antibody 66387-1-IgDownload protocol
IF protocol for GSDMD antibody 66387-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cancer

Metal-enriched HSP90 nanoinhibitor overcomes heat resistance in hyperthermic intraperitoneal chemotherapy used for peritoneal metastases

Authors - Qiang Wang
View Article
humanWB

PLoS Pathog

An NLRP3 inflammasome-triggered cytokine storm contributes to Streptococcal toxic shock-like syndrome (STSLS).

Authors - Lan Lin
View Article
humanWB

iScience

ASC/inflammasome-independent pyroptosis in ovarian cancer cells through translational augmentation of caspase-1

Authors - Ozlem Calbay
View Article
humanWB

Emerg Microbes Infect

Acquiring high expression of suilysin enable non-epidemic Streptococccus suis to cause Streptococcal toxic shock-like syndrome (STSLS) through NLRP3 inflammasome hyperactivation.

Authors - Lei Xu
View Article
mouseWB

Mol Med

MALAT1 knockdown alleviates the pyroptosis of microglias in diabetic cerebral ischemia via regulating STAT1 mediated NLRP3 transcription

Authors - Nan Zhao
View Article
mouseWB,IF

J Ethnopharmacol

Xiaoqinglong decoction improves allergic rhinitis by inhibiting NLRP3-mediated pyroptosis in BALB/C mice

Authors - Ruizhi Wang
View Article