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  • Featured Product
  • KD/KO Validated

GUK1 Monoclonal antibody

GUK1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H5E1

Cat no : 67047-1-Ig

Synonyms

GMK, GMP kinase, Guanylate kinase, guanylate kinase 1, GUK1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of Jurkat using 67047-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of HEK-293 using 67047-1-Ig

    WB result of GUK1 antibody (67047-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUK1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HeLa cells using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of HeLa using 67047-1-Ig

    WB result of GUK1 antibody (67047-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUK1 transfected HeLa cells.

  • Western Blot (WB) analysis of HEK-293 cells using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of HEK-293 using 67047-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of fetal human brain using 67047-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of rat brain using 67047-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of mouse brain using 67047-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using GUK1 Monoclonal antibody (67047-1-Ig)

    WB analysis of Jurkat using 67047-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67047-1-Ig (GUK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    IHC staining of human thyroid cancer using 67047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67047-1-Ig (GUK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    IHC staining of human thyroid cancer using 67047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 67047-1-Ig (GUK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    IHC staining of human prostate cancer using 67047-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67047-1-Ig (GUK1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human thyroid cancer tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    IF Staining of human thyroid cancer using 67047-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using GUK1 antibody (67047-1-Ig, Clone: 2H5E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human thyroid cancer tissue using GUK1 Monoclonal antibody (67047-1-Ig)

    IF Staining of human thyroid cancer using 67047-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using GUK1 antibody (67047-1-Ig, Clone: 2H5E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, fetal human brain tissue, rat brain tissue, mouse brain tissue, HeLa cells
Positive IHC detected inhuman thyroid cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman thyroid cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67047-1-Ig targets GUK1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GUK1 fusion protein Ag28805
Full Name guanylate kinase 1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22-27 kDa
GenBank Accession NumberBC009914
Gene Symbol GUK1
Gene ID (NCBI) 2987
RRIDAB_2882360
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GUK1 antibody 67047-1-IgDownload protocol
IHC protocol for GUK1 antibody 67047-1-IgDownload protocol
IF protocol for GUK1 antibody 67047-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols