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  • KD/KO Validated

GUSB Polyclonal antibody

GUSB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16332-1-AP

Synonyms

Beta G1, Beta glucuronidase, glucuronidase, beta, GUSB, MPS7



Tested Applications

Positive WB detected inHEK-293T cells, mouse liver tissue, HL-60 cells, mouse heart tissue, K-562 cells, rat liver tissue
Positive IHC detected inhuman colon cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16332-1-AP targets GUSB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GUSB fusion protein Ag9453
Full Name glucuronidase, beta
Calculated Molecular Weight 651 aa, 75 kDa
Observed Molecular Weight 78 kDa
GenBank Accession NumberBC014142
Gene Symbol GUSB
Gene ID (NCBI) 2990
RRIDAB_2279538
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The GUSB gene encodes beta-glucuronidase, a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans. It is a tetrameric glycoprotein composed of identical subunits. GUSB plays an important role in the degradation of dermatan and keratan sulfates. GUSB has two isoforms with the molecular mass of 75 kDa and 69 kDa, and it always can be detected as 78 kDa, 60 kDa and 18 kDa. The 60 kDa and 18 kDa polypeptides are derived by nicking 78-kDa GUSB subunit at Val159-Gly160 (PMID: 1311180, 9268591).

Protocols

Product Specific Protocols
WB protocol for GUSB antibody 16332-1-APDownload protocol
IHC protocol for GUSB antibody 16332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Science

The adaptive immune system is a major driver of selection for tumor suppressor gene inactivation.

Authors - Timothy D Martin
  • KO Validated
mouseIHC

J Invest Dermatol

Distinct roles for Dectin-1 and Dectin-2 in skin wound healing and neutrophilic inflammatory responses.

Authors - Kenji Yamaguchi
humanWB

PLoS One

Changes in retinal pigment epithelium related to cigarette smoke: possible relevance to smoking as a risk factor for age-related macular degeneration.

Authors - Wang Ai Ling AL
mouseWB

PLoS One

Mitochondrial dysfunction leads to deconjugation of quercetin glucuronides in inflammatory macrophages.

Authors - Ishisaka Akari A
mouseWB

PLoS One

C-Type Lectin Receptor Dectin-2 Binds to an Endogenous Protein β-Glucuronidase on Dendritic Cells.

Authors - Daiki Mori
  • KO Validated
mouseWB

Gut Microbes

Vancomycin relieves tacrolimus-induced hyperglycemia by eliminating gut bacterial beta-glucuronidase enzyme activity

Authors - Peixia Li