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HAI-1 Polyclonal antibody

HAI-1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27593-1-AP

Synonyms

HAI, HAI 1, HAI1, MANSC2, SPINT1

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using HAI-1 Polyclonal antibody (27593-1-AP)

    WB analysis of human placenta using 27593-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27593-1-AP (HAI-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using HAI-1 Polyclonal antibody (27593-1-AP)

    WB analysis of MCF-7 using 27593-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27593-1-AP (HAI-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HAI-1 Polyclonal antibody (27593-1-AP)

    IHC staining of human lung cancer using 27593-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27593-1-AP (HAI-1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HAI-1 Polyclonal antibody (27593-1-AP)

    IHC staining of human lung cancer using 27593-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27593-1-AP (HAI-1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman placenta tissue, MCF-7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27593-1-AP targets HAI-1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HAI-1 fusion protein Ag26733
Full Name serine peptidase inhibitor, Kunitz type 1
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC004140
Gene Symbol SPINT1
Gene ID (NCBI) 6692
RRIDAB_2880918
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HAI-1 antibody 27593-1-APDownload protocol
IHC protocol for HAI-1 antibody 27593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols