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HAI-1 Monoclonal antibody

HAI-1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2H2D8

Cat no : 67031-1-Ig

Synonyms

HAI, HAI 1, HAI1, HAI-1, MANSC2, SPINT1

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using HAI-1 Monoclonal antibody (67031-1-Ig)

    WB analysis of human placenta using 67031-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67031-1-Ig (HAI-1 antibody) at dilution of 1:900 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HAI-1 Monoclonal antibody (67031-1-Ig)

    IF Staining of MCF-7 using 67031-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using HAI-1 antibody (67031-1-Ig, Clone: 2H2D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67031-1-Ig targets HAI-1 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HAI-1 fusion protein Ag26733
Full Name serine peptidase inhibitor, Kunitz type 1
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC004140
Gene Symbol SPINT1
Gene ID (NCBI) 6692
RRIDAB_2882346
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HAI-1 antibody 67031-1-IgDownload protocol
IF protocol for HAI-1 antibody 67031-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols