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HAL Polyclonal antibody

HAL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25940-1-AP

Synonyms

HAL, HIS, Histidase, histidine ammonia lyase, HSTD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HAL Polyclonal antibody (25940-1-AP)

    WB analysis using 25940-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25940-1-AP (HAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using HAL Polyclonal antibody (25940-1-AP)

    IHC staining of human skin using 25940-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 25940-1-AP (HAL Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using HAL Polyclonal antibody (25940-1-AP)

    IHC staining of mouse skin using 25940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 25940-1-AP (HAL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using HAL Polyclonal antibody (25940-1-AP)

    IHC staining of mouse skin using 25940-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 25940-1-AP (HAL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman skin tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25940-1-AP is HAL Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HAL fusion protein Ag22985
Full Name histidine ammonia-lyase
Calculated Molecular Weight 657 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC096097
Gene Symbol HAL
Gene ID (NCBI) 3034
RRIDAB_2880303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HAL, also named as Histidase and HIS, catalyzes the nonoxidative elimination of the α-amino group of histidine. It is closely related to the important plant enzyme phenylalanine ammonia-lyase. HAL has some isoforms with MW 73 kDa, 65 kDa and 49 kDa.

Protocols

Product Specific Protocols
WB protocol for HAL antibody 25940-1-APDownload protocol
IHC protocol for HAL antibody 25940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Autophagy

The Valsa mali effector Vm1G-1794 protects the aggregated MdEF-Tu from autophagic degradation to promote infection in apple

Authors - Runmin Che
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Hua (Verified Customer) (05-20-2022)

Very good antibody. Strong signal and clean background

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: mouse liver
HAL Antibody Western Blot validation (1:4000 dilution) in mouse liver (Cat no:25940-1-AP)