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HGSNAT Polyclonal antibody

HGSNAT Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12399-1-AP

Synonyms

HGNAT, HGSNAT, MPS3C, TMEM76, Transmembrane protein 76

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using HGSNAT Polyclonal antibody (12399-1-AP)

    WB analysis of HepG2 using 12399-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12399-1-AP (HGSNAT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HGSNAT Polyclonal antibody (12399-1-AP)

    WB analysis using 12399-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12399-1-AP (HGSNAT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HGSNAT Polyclonal antibody (12399-1-AP)

    WB analysis using 12399-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12399-1-AP (HGSNAT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using HGSNAT Polyclonal antibody (12399-1-AP)

    WB analysis of mouse liver using 12399-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12399-1-AP (HGSNAT Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using HGSNAT Polyclonal antibody (12399-1-AP)

    WB analysis of mouse liver using 12399-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12399-1-AP (HGSNAT Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HGSNAT Polyclonal antibody (12399-1-AP)

    IHC staining of human lung cancer using 12399-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12399-1-AP (HGSNAT antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HGSNAT Polyclonal antibody (12399-1-AP)

    IF Staining of HepG2 using 12399-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12399-1-AP (HGSNAT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, A549 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12399-1-AP targets HGSNAT in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HGSNAT fusion protein Ag3089
Full Name heparan-alpha-glucosaminide N-acetyltransferase
Calculated Molecular Weight 206 aa, 22 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC012452
Gene Symbol HGSNAT
Gene ID (NCBI) 138050
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HGSNAT antibody 12399-1-APDownload protocol
IHC protocol for HGSNAT antibody 12399-1-APDownload protocol
IF protocol for HGSNAT antibody 12399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols