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HIST2H2AA4 Polyclonal antibody

HIST2H2AA4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15302-1-AP

Synonyms

H2A/R, H2AFO, HIST2H2AA, HIST2H2AA3, HIST2H2AA4, histone, histone cluster 2, H2aa4, Histone H2A type 2 A, Histone H2A.2, Histone H2A/o

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using HIST2H2AA4 Polyclonal antibody (15302-1-AP)

    WB analysis of L02 using 15302-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of Jurkat cells using HIST2H2AA4 Polyclonal antibody (15302-1-AP)

    WB analysis of Jurkat using 15302-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15302-1-AP (HIST2H2AA4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using HIST2H2AA4 Polyclonal antibody (15302-1-AP)

    WB analysis of K-562 using 15302-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15302-1-AP (HIST2H2AA4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using HIST2H2AA4 Polyclonal antibody (15302-1-AP)

    WB analysis of mouse liver using 15302-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using HIST2H2AA4 Polyclonal antibody (15302-1-AP)

    WB analysis of rat liver using 15302-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using HIST2H2AA4 Polyclonal antibody (15302-1-AP)

    WB analysis of mouse liver using 15302-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HIST2H2AA4 Polyclonal antibody (15302-1-AP)

    IHC staining of human liver cancer using 15302-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HIST2H2AA4 Polyclonal antibody (15302-1-AP)

    IHC staining of human liver cancer using 15302-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inL02 cells, Jurkat cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15302-1-AP targets HIST2H2AA4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIST2H2AA4 fusion protein Ag7560
Full Name histone cluster 2, H2aa4
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC001629
Gene Symbol HIST2H2AA4
Gene ID (NCBI) 723790
RRIDAB_2878124
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HIST2H2AA4 antibody 15302-1-APDownload protocol
IHC protocol for HIST2H2AA4 antibody 15302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Physiol Biochem

Effect of Type I Diabetes on the Proteome of Mouse Oocytes.

Authors - Guangjian Jiang
View Article