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HMCN1 Polyclonal antibody

HMCN1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18837-1-AP

Synonyms

ARMD1, FBLN6, FIBL 6, FIBL6, Fibulin 6, hemicentin 1, HMCN1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skin tissue using HMCN1 Polyclonal antibody (18837-1-AP)

    WB analysis of mouse skin using 18837-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 18837-1-AP (HMCN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using HMCN1 Polyclonal antibody (18837-1-AP)

    IHC staining of human colon using 18837-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 18837-1-AP (HMCN1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using HMCN1 Polyclonal antibody (18837-1-AP)

    IHC staining of human colon using 18837-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 18837-1-AP (HMCN1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using HMCN1 Polyclonal antibody (18837-1-AP)

    IF Staining of hTERT-RPE1 using 18837-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using HMCN1 antibody (18837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HMCN1 Polyclonal antibody (18837-1-AP)

    IF Staining of MCF-7 using 18837-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HMCN1 antibody (18837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse skin tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18837-1-AP targets HMCN1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name hemicentin 1
Calculated Molecular Weight 613 kDa, 600 kDa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberNM_031935
Gene Symbol HMCN1
Gene ID (NCBI) 83872
RRIDAB_2878563
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hemicentin-1 (HMCN1), also known as fibulin-6 (FIBL-6), is an extracellular protein of the immunoglobulin superfamily. HMCN1 consists of a von Willebrand A domain, tandem immunoglobulin modules, tandem EGF modules, and a fibulin carboxy-terminal module (PMID: 24912920). Mutations of HMCN1 gene may be associated with age-related macular degeneration. Three alternative transcripts encoding different HMCN1 isoforms have been described. This antibody raised against a synthesized peptide corresponding to 960-916 amino acids of human HMCN1 can recognize all three isoforms of HMCN1.

Protocols

Product Specific Protocols
WB protocol for HMCN1 antibody 18837-1-APDownload protocol
IHC protocol for HMCN1 antibody 18837-1-APDownload protocol
IF protocol for HMCN1 antibody 18837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Front Pharmacol

Artemisinin relieves myocardial ischemia-reperfusion injury via modulating miR-29b-3p and hemicentin 1

Authors - Junyu Han
View Article