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- KD/KO Validated
HNF1A Polyclonal antibody
HNF1A Polyclonal Antibody for IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IP, IHC, CoIP, chIP, ELISA
Conjugate
Unconjugated
Cat no : 22426-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | rat liver tissue, SMMC-7721 cells |
| Positive IP detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
| CoIP | See 3 publications below |
| ChIP | See 2 publications below |
Product Information
The immunogen of 22426-1-AP is HNF1A Fusion Protein expressed in E. coli.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HNF1A fusion protein Ag18188 |
| Full Name | HNF1 homeobox A |
| Calculated Molecular Weight | 631 aa, 67 kDa |
| Observed Molecular Weight | 67 kDa |
| GenBank Accession Number | BC104910 |
| Gene Symbol | HNF1A |
| Gene ID (NCBI) | 6927 |
| RRID | AB_2879104 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HNF-1 , a homoeprotein family designated the Hepatocyte Nuclear Factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF1 as a transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. It is required for the expression of several liver specific genes and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HNF1A antibody 22426-1-AP | Download protocol |
| IP protocol for HNF1A antibody 22426-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer. | ||
Mol Ther Nucleic Acids The accessible promoter-mediated supplementary effect of host factors provides new insight into the tropism of SARS-CoV-2. | ||
Acta Pharmacol Sin Aloe-emodin exerts cholesterol-lowering effects by inhibiting proprotein convertase subtilisin/kexin type 9 in hyperlipidemic rats. | ||
Chem Biol Interact 6-Formylindolo(3,2-b)carbazole induced aryl hydrocarbon receptor activation prevents intestinal barrier dysfunction through regulation of claudin-2 expression.
| ||
J Exp Clin Cancer Res ROS/PI3K/Akt and Wnt/β-catenin signalings activate HIF-1α-induced metabolic reprogramming to impart 5-fluorouracil resistance in colorectal cancer. | ||
Cell Death Dis CBP/p300 HAT maintains the gene network critical for β cell identity and functional maturity. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kamal (Verified Customer) (07-05-2023) | Works with 1X TBST for WB.
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