• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

HNRNPF Polyclonal antibody

HNRNPF Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14974-1-AP

Synonyms

hnRNP F, HNRNPF, HNRPF, mcs94 1, Nucleolin like protein mcs94 1, OK/SW cl.23

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using HNRNPF Polyclonal antibody (14974-1-AP)

    WB analysis of HeLa using 14974-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14974-1-AP (HNRNPF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HNRNPF Polyclonal antibody (14974-1-AP)

    WB analysis of HeLa using 14974-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14974-1-AP (HNRNPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using HNRNPF Polyclonal antibody (14974-1-AP)

    WB analysis of SH-SY5Y using 14974-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14974-1-AP (HNRNPF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HNRNPF Polyclonal antibody (14974-1-AP)

    IHC staining of human colon cancer using 14974-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14974-1-AP (HNRNPF antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HNRNPF Polyclonal antibody (14974-1-AP)

    IHC staining of human colon cancer using 14974-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14974-1-AP (HNRNPF antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HNRNPF Polyclonal antibody (14974-1-AP)

    IF Staining of HeLa using 14974-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14974-1-AP (HNRNPF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14974-1-AP targets HNRNPF in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPF fusion protein Ag6885
Full Name heterogeneous nuclear ribonucleoprotein F
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC001432
Gene Symbol HNRNPF
Gene ID (NCBI) 3185
RRIDAB_2263984
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRNPF, also named as Heterogeneous nuclear ribonucleoprotein F,is a 415 amino acid portein, which contains 3 RRM (RNA recognition motif) domains. HNRNPF is expressed ubiquitously in various tissues and localizes in the nucleus. HNRNPF is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPF plays a role in the regulation of alternative splicing events.

Protocols

Product Specific Protocols
WB protocol for HNRNPF antibody 14974-1-APDownload protocol
IHC protocol for HNRNPF antibody 14974-1-APDownload protocol
IF protocol for HNRNPF antibody 14974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mar Drugs

An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells.

Authors - Su Ching-Chyuan CC
View Article
humanWB,IF,IP

Virol J

Interaction of HnRNP F with the guanine-rich segments in viral antigenomic RNA enhances porcine reproductive and respiratory syndrome virus-2 replication.

Authors - Aiguo Zhang
  • KD Validated
View Article
humanIHC

Front Oncol

The hnRNPK/A1/R/U Complex Regulates Gene Transcription and Translation and is a Favorable Prognostic Biomarker for Human Colorectal Adenocarcinoma.

Authors - Yixin Li
View Article