• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HNRPLL Polyclonal antibody

HNRPLL Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26769-1-AP

Synonyms

hnRNPLL, HNRPLL, SRRF, Stromal RNA regulating factor

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HNRPLL Polyclonal antibody (26769-1-AP)

    WB analysis using 26769-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26769-1-AP (HNRPLL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HNRPLL Polyclonal antibody (26769-1-AP)

    WB analysis using 26769-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26769-1-AP (HNRPLL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using HNRPLL Polyclonal antibody (26769-1-AP)

    IHC staining of mouse kidney using 26769-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26769-1-AP (HNRPLL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HNRPLL Polyclonal antibody (26769-1-AP)

    IHC staining of human stomach cancer using 26769-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26769-1-AP (HNRPLL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using HNRPLL Polyclonal antibody (26769-1-AP)

    IHC staining of human stomach cancer using 26769-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26769-1-AP (HNRPLL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using HNRPLL Polyclonal antibody (26769-1-AP)

    IHC staining of mouse kidney using 26769-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26769-1-AP (HNRPLL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HNRPLL Polyclonal antibody (26769-1-AP)

    IF Staining of HepG2 using 26769-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26769-1-AP (HNRPLL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, K-562 cells
Positive IHC detected inmouse kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26769-1-AP is HNRPLL Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRPLL fusion protein Ag25287
Full Name heterogeneous nuclear ribonucleoprotein L-like
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC017480
Gene Symbol HNRPLL
Gene ID (NCBI) 92906
RRIDAB_2880628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRPLL, also named as HNRNPLL and SRRF, is an RNA binding protein that regulates alternative splicing of pre-mRNA. HnRNPLL is up-regulated in stimulated T cells, bound CD45 transcripts, and is a key inducible regulator of CD45 alternative splicing (PMID: 18669861). HNRPLL has 5 isoforms with the molecular weights of 31-60 kDa. Additionally, studies have shown that HNRPLL is a metastasis suppressor gene in colorectal cancer (PMID: 28360095).

Protocols

Product Specific Protocols
WB protocol for HNRPLL antibody 26769-1-APDownload protocol
IHC protocol for HNRPLL antibody 26769-1-APDownload protocol
IF protocol for HNRPLL antibody 26769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Stem Cell Rev Rep

Lnc-PPP2R1B Mediates the Alternative Splicing of PPP2R1B by Interacting and Stabilizing HNRNPLL and Promotes Osteogenesis of MSCs

Authors - Shuping Peng
View Article
humanWB,IHC,IF

J Exp Clin Cancer Res

Energy stress-induced circZFR enhances oxidative phosphorylation in lung adenocarcinoma via regulating alternative splicing

Authors - Zhifei Ma
  • KD Validated
View Article