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HOXA2 Polyclonal antibody

HOXA2 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 25044-1-AP

Synonyms

homeobox A2, Homeobox protein Hox 1K, Homeobox protein Hox A2, HOX1K, HOXA2

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using HOXA2 Polyclonal antibody (25044-1-AP)

    WB analysis of HepG2 using 25044-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25044-1-AP (HOXA2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of C6 cells using HOXA2 Polyclonal antibody (25044-1-AP)

    WB analysis of C6 using 25044-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 25044-1-AP (HOXA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using HOXA2 Polyclonal antibody (25044-1-AP)

    WB analysis of Jurkat using 25044-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25044-1-AP (HOXA2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, C6 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25044-1-AP is HOXA2 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOXA2 fusion protein Ag19224
Full Name homeobox A2
Calculated Molecular Weight 376 aa, 41 kDa
Observed Molecular Weight 41-43 kDa
GenBank Accession NumberBC130571
Gene Symbol HOXA2
Gene ID (NCBI) 3199
RRIDAB_2879868
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOXA2, also named as Homeobox protein Hox-A2, is a 376 amino acid protein, which contains 1 homeobox DNA-binding domain and belongs to the Antp homeobox family. HOXA2 localizes in the nucleus and hsa an association with Microtia, hearing impairment, and cleft palate. HOXA2 as a sequence-specific transcription factor is part of a developmental regulatory system, which provides cells with specific positional identities on the anterior-posterior axis.

Protocols

Product Specific Protocols
WB protocol for HOXA2 antibody 25044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols