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HPCAL4 Polyclonal antibody

HPCAL4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12470-1-AP

Synonyms

hippocalcin like 4, Hippocalcin like protein 4, HLP4, HPCAL4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HPCAL4 Polyclonal antibody (12470-1-AP)

    WB analysis using 12470-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12470-1-AP (HPCAL4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using HPCAL4 Polyclonal antibody (12470-1-AP)

    WB analysis of human brain using 12470-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12470-1-AP (HPCAL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of fetal human brain tissue using HPCAL4 Polyclonal antibody (12470-1-AP)

    IP experiment of fetal human brain using 12470-1-AP

    IP result of anti-HPCAL4 (IP:12470-1-AP, 4ug; Detection:12470-1-AP 1:700) with fetal human brain tissue lysate 3200 ug.

  • Immunohistochemistry (IHC) staining of human medulloblastoma tissue using HPCAL4 Polyclonal antibody (12470-1-AP)

    IHC staining of human medulloblastoma using 12470-1-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12470-1-AP (HPCAL4 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected infetal human brain tissue, human brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected infetal human brain tissue
Positive IHC detected inhuman medulloblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12470-1-AP targets HPCAL4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HPCAL4 fusion protein Ag3153
Full Name hippocalcin like 4
Calculated Molecular Weight 191 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC030827
Gene Symbol HPCAL4
Gene ID (NCBI) 51440
RRIDAB_2120231
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HPCAL4 antibody 12470-1-APDownload protocol
IHC protocol for HPCAL4 antibody 12470-1-APDownload protocol
IP protocol for HPCAL4 antibody 12470-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols