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  • Featured Product
  • KD/KO Validated

HPS6 Polyclonal antibody

HPS6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11371-1-AP

Synonyms

Hermansky Pudlak syndrome 6, HPS6, RP11 302K17.1, Ru, Ruby eye protein homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HPS6 Polyclonal antibody (11371-1-AP)

    WB analysis of HeLa using 11371-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11371-1-AP (HPS6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using HPS6 Polyclonal antibody (11371-1-AP)

    WB analysis of MCF-7 using 11371-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11371-1-AP (HPS6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HPS6 Polyclonal antibody (11371-1-AP)

    WB analysis of HEK-293 using 11371-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11371-1-AP (HPS6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using HPS6 Polyclonal antibody (11371-1-AP)

    IP experiment of HeLa using 11371-1-AP

    IP result of anti-HPS6 (IP:11371-1-AP, 4ug; Detection:11371-1-AP 1:300) with HeLa cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HPS6 Polyclonal antibody (11371-1-AP)

    IHC staining of human ovary tumor using 11371-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11371-1-AP (HPS6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HPS6 Polyclonal antibody (11371-1-AP)

    IHC staining of human ovary tumor using 11371-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11371-1-AP (HPS6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HPS6 Polyclonal antibody (11371-1-AP)

    IHC staining of human kidney using 11371-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11371-1-AP (HPS6 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HPS6 Polyclonal antibody (11371-1-AP)

    IHC staining of human kidney using 11371-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11371-1-AP (HPS6 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11371-1-AP targets HPS6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HPS6 fusion protein Ag1929
Full Name Hermansky-Pudlak syndrome 6
Calculated Molecular Weight 775 aa, 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC011594
Gene Symbol HPS6
Gene ID (NCBI) 79803
RRIDAB_2120693
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hermansky-Pudlak syndrome (HPS) defines a group of at least seven autosomal recessive disorders characterized by albinism and prolonged bleeding due to defects in the lysosome-related organelles, melanosomes and platelet-dense granules, respectively. HSP6, also named as Ru, regulates the synthesis and function of lysosomes and of highly specialized organelles, such as melanosomes and platelet dense granules. It acts as cargo adapter for the dynein-dynactin motor complex to mediate the transport of lysosomes from the cell periphery to the perinuclear region.

Protocols

Product Specific Protocols
WB protocol for HPS6 antibody 11371-1-APDownload protocol
IHC protocol for HPS6 antibody 11371-1-APDownload protocol
IP protocol for HPS6 antibody 11371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Blood

vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.

Authors - Anish Vaibhav Sharda
  • KD Validated
View Article
humanWB

EMBO Rep

Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes.

Authors - Saurabh Shakya
View Article
mouseWB

Am J Respir Cell Mol Biol

Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes.

Authors - Seunghyi Kook
View Article