HRSP12 Polyclonal antibody
HRSP12 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12930-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
12930-1-AP targets HRSP12 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HRSP12 fusion protein Ag3628 |
| Full Name | heat-responsive protein 12 |
| Calculated Molecular Weight | 14.5 kDa |
| Observed Molecular Weight | 14.5 kDa |
| GenBank Accession Number | BC010280 |
| Gene Symbol | HRSP12 |
| Gene ID (NCBI) | 10247 |
| RRID | AB_2119169 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribonuclease UK114, also named HRSP12, is found predominantly in human adult kidney and liver and is responsible for inhibiting translation by cleaving mRNA. UK114 is mostly expressed in cytoplasm, but occasionally in nucleus in less differentiated cells. UK114 cleaves phosphodiester bonds only in single-stranded RNA. The levels of both the mRNA and protein are markedly reduced in heptatocellular tumors and in human hepatoma cell lines compared with normal liver tissues, suggesting that ribonuclease UK114 may be an important biomarker for heptatic carcinoma. Current antibody catalog# 12930-1-AP is a rabbit polyclonal antibody raised from human full-length UK114.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HRSP12 antibody 12930-1-AP | Download protocol |
| IHC protocol for HRSP12 antibody 12930-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Biol Y-box binding protein 1 and RNase UK114 mediate monocyte chemoattractant protein 1 mRNA stability in vascular smooth muscle cells. | ||
Genes Genomics P14.5 functions to inhibit cell migration and can be used as a prognostic marker in hepatocellular carcinoma |
