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IAH1 Polyclonal antibody

IAH1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17808-1-AP

Synonyms

IAH1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse testis tissue using IAH1 Polyclonal antibody (17808-1-AP)

    WB analysis of mouse testis using 17808-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17808-1-AP (IAH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using IAH1 Polyclonal antibody (17808-1-AP)

    WB analysis of mouse brain using 17808-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17808-1-AP (IAH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using IAH1 Polyclonal antibody (17808-1-AP)

    WB analysis of mouse kidney using 17808-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17808-1-AP (IAH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using IAH1 Polyclonal antibody (17808-1-AP)

    IP experiment of mouse kidney using 17808-1-AP

    IP result of anti-IAH1 (IP:17808-1-AP, 3ug; Detection:17808-1-AP 1:500) with mouse kidney tissue lysate 5000ug.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using IAH1 Polyclonal antibody (17808-1-AP)

    IP experiment of mouse testis using 17808-1-AP

    IP result of anti-IAH1 (IP:17808-1-AP, 3ug; Detection:17808-1-AP 1:500) with mouse testis tissue lysate 4800ug.

Tested Applications

Positive WB detected inmouse testis tissue, mouse brain tissue, mouse kidney tissue
Positive IP detected inmouse kidney tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17808-1-AP targets IAH1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IAH1 fusion protein Ag12089
Full Name isoamyl acetate-hydrolyzing esterase 1 homolog (S. cerevisiae)
Calculated Molecular Weight 248 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC111025
Gene Symbol IAH1
Gene ID (NCBI) 285148
RRIDAB_2123524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IAH1 antibody 17808-1-APDownload protocol
IP protocol for IAH1 antibody 17808-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Proteomics

Increased expression of peroxiredoxin 1 and identification of a novel lipid-metabolizing enzyme in the early phase of liver ischemia reperfusion injury.

Authors - Wilson Claire H CH
View Article