IER2 Polyclonal antibody

IER2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23849-1-AP

Synonyms

3CH92, CHX1, ETR101, IER2, immediate early response 2, Pip92, Protein ETR101



Tested Applications

Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 23849-1-AP is IER2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IER2 fusion protein Ag20869
Full Name immediate early response 2
Calculated Molecular Weight 223 aa, 24 kDa
GenBank Accession NumberBC072432
Gene Symbol IER2
Gene ID (NCBI) 9592
RRIDAB_2879339
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ier2 (also called ETR101, Pip92, 3CH92 and CHX1, hereafter referred to as Ier2, regardless of species) is an immediate early gene (PMID: 2678945) that was originally found to be upregulated in fibroblasts upon their activation (PMID: 3841511). Immediate early genes are a group of genes that are rapidly induced in quiescent cells by proliferation- and migration-inducing stimuli. Ier2 may have a role in tumor cell motility and metastasis (PMID: 22120713).

Protocols

Product Specific Protocols
IHC protocol for IER2 antibody 23849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Signal

IER family proteins are regulators of protein phosphatase PP2A and modulate the phosphorylation status of CDC25A.

Authors - Takumi Ueda
humanWB

FEBS J

PP2A-B55 and its adapter proteins IER2 and IER5 regulate the activity of RB family proteins and the expression of cell cycle-related genes

Authors - Kuriko Doi