• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

IGHMBP2 Polyclonal antibody

IGHMBP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23945-1-AP

Synonyms

ATP dependent helicase IGHMBP2, CATF1, DNA binding protein SMUBP 2, GF 1, Glial factor 1, HCSA, HMN6, IGHMBP2, SMARD1, SMBP2, SMUBP2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using IGHMBP2 Polyclonal antibody (23945-1-AP)

    WB analysis using 23945-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23945-1-AP (IGHMBP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using IGHMBP2 Polyclonal antibody (23945-1-AP)

    WB analysis of human brain using 23945-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 23945-1-AP (IGHMBP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IGHMBP2 Polyclonal antibody (23945-1-AP)

    IHC staining of human liver cancer using 23945-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23945-1-AP (IGHMBP2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using IGHMBP2 Polyclonal antibody (23945-1-AP)

    IHC staining of human liver cancer using 23945-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23945-1-AP (IGHMBP2 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inHL-60 cells, human brain tissue, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 23945-1-AP is IGHMBP2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGHMBP2 fusion protein Ag21057
Full Name immunoglobulin mu binding protein 2
Calculated Molecular Weight 993 aa, 109 kDa
Observed Molecular Weight 109 kDa
GenBank Accession NumberBC105088
Gene Symbol IGHMBP2
Gene ID (NCBI) 3508
RRIDAB_2879371
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGHMBP2 also named as immunoglobulin mu binding protein 2 is a 993 amino-acid protein, which localizes in nuclear and cytoplasm. The molecular weight of IGHMBP2 is 109 kDa. IGHMBP2 belongs to the DNA2/NAM7 helicase family. IGHMBP2 also acts as a transcriptional regulator and is necessary for transcriptional activation of the flounder liver-type antifreeze protein gene. IGHMBP2 exists as a homooligomer and is part of the cytosolic ribonucleoprotein complex.

Protocols

Product Specific Protocols
WB protocol for IGHMBP2 antibody 23945-1-APDownload protocol
IHC protocol for IGHMBP2 antibody 23945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

bioRxiv

IGHMBP2 deletion suppresses translation and activates the integrated stress response

Authors - Jesslyn E Park
View Article