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  • Featured Product
  • KD/KO Validated

IGSF21 Polyclonal antibody

IGSF21 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21465-1-AP

Synonyms

IGSF21

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using IGSF21 Polyclonal antibody (21465-1-AP)

    WB analysis of mouse brain using 21465-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21465-1-AP (IGSF21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21465-1-AP is IGSF21 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGSF21 fusion protein Ag15062
Full Name immunoglobin superfamily, member 21
Calculated Molecular Weight 467 aa, 52 kDa
Observed Molecular Weight 48-50 kDa
GenBank Accession NumberBC007618
Gene Symbol IGSF21
Gene ID (NCBI) 84966
RRIDAB_10734429
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGSF21 (Immunoglobulin superfamily member 21) is a GPI-anchored protein that contains two immunoglobulin-like domains (Ig-like 1 and Ig-like 2). The Ig-like 1 domain of IGSF21 is indispensable for synaptogenic activity whereas its Ig-like 2 domain is secondarily responsible for the activity. It has been reported that IGSF21 selectively regulates inhibitory presynaptic differentiation through interacting with presynaptic neurexin2α and plays a crucial role in synaptic inhibition in the brain. (PMID: 28864826)

Protocols

Product Specific Protocols
WB protocol for IGSF21 antibody 21465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2α.

Authors - Yuko Tanabe
  • KO Validated
View Article