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IIP45 Polyclonal antibody

IIP45 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20630-1-AP

Synonyms

IGFBP2 binding protein, IIP45, invasion inhibitory protein 45, MIIP

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using IIP45 Polyclonal antibody (20630-1-AP)

    WB analysis of fetal human brain using 20630-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 20630-1-AP (IIP45 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using IIP45 Polyclonal antibody (20630-1-AP)

    IHC staining of human liver using 20630-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20630-1-AP (IIP45 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using IIP45 Polyclonal antibody (20630-1-AP)

    IHC staining of human liver using 20630-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20630-1-AP (IIP45 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using IIP45 Polyclonal antibody (20630-1-AP)

    IHC staining of human brain using 20630-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20630-1-AP (IIP45 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using IIP45 Polyclonal antibody (20630-1-AP)

    IHC staining of human brain using 20630-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20630-1-AP (IIP45 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected infetal human brain tissue
Positive IHC detected inhuman liver tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20630-1-AP is IIP45 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IIP45 fusion protein Ag14609
Full Name invasion inhibitory protein 45
Calculated Molecular Weight 388 aa, 43 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC008068
Gene Symbol IIP45
Gene ID (NCBI) 60672
RRIDAB_2878712
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IIP45 antibody 20630-1-APDownload protocol
IHC protocol for IIP45 antibody 20630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta

GAS5 suppresses malignancy of human glioma stem cells via a miR-196a-5p/FOXO1 feedback loop.

Authors - Xihe Zhao
View Article
humanIHC

Cancer Biol Med

MIIP inhibits clear cell renal cell carcinoma proliferation and angiogenesis via negative modulation of the HIF-2α-CYR61 axis

Authors - Fengqi Yan
View Article