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IL-15RA Monoclonal antibody

IL-15RA Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

5E3E1

Cat no : 60138-1-Ig

Synonyms

IL 15 receptor subunit alpha, IL 15R alpha, IL 15RA, IL15RA, IL-15RA, interleukin 15 receptor, alpha, sIL 15 receptor subunit alpha, sIL 15R alpha, sIL 15RA

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using IL-15RA Monoclonal antibody (60138-1-Ig)

    WB analysis of Raji using 60138-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 60138-1-Ig (IL15RA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Daudi cells using IL-15RA Monoclonal antibody (60138-1-Ig)

    WB analysis of Daudi using 60138-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 60138-1-Ig (IL15RA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using IL-15RA Monoclonal antibody (60138-1-Ig)

    WB analysis of HeLa using 60138-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60138-1-Ig (IL15RA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inRaji cells, Daudi cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60138-1-Ig targets IL-15RA in WB,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-15RA fusion protein Ag10200
Full Name interleukin 15 receptor, alpha
Calculated Molecular Weight 231 aa, 24 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC107777
Gene Symbol IL15RA
Gene ID (NCBI) 3601
RRIDAB_2881352
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897). The calculated molecular weight of full-length IL-15Rα is 28 kDa, IL-15Rα can be glycosylated, and larger apparent molecular weights of 55-65 kDa have been reported by some researches (PMID: 10480910; 15976182; 15671076; 17912462).

Protocols

Product Specific Protocols
WB protocol for IL-15RA antibody 60138-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols