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ILVBL Polyclonal antibody

ILVBL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24738-1-AP

Synonyms

209L8, AHAS, ILV2H, IlvB like protein, ILVBL

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ILVBL Polyclonal antibody (24738-1-AP)

    WB analysis of HepG2 using 24738-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24738-1-AP (ILVBL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ILVBL Polyclonal antibody (24738-1-AP)

    WB analysis of MCF-7 using 24738-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24738-1-AP (ILVBL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using ILVBL Polyclonal antibody (24738-1-AP)

    IHC staining of human heart using 24738-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24738-1-AP (ILVBL Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ILVBL Polyclonal antibody (24738-1-AP)

    IHC staining of human heart using 24738-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24738-1-AP (ILVBL Antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24738-1-AP is ILVBL Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ILVBL fusion protein Ag21495
Full Name ilvB (bacterial acetolactate synthase)-like
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC011761
Gene Symbol ILVBL
Gene ID (NCBI) 10994
RRIDAB_2879699
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ILVBL antibody 24738-1-APDownload protocol
IHC protocol for ILVBL antibody 24738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols