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INTS9 Polyclonal antibody

INTS9 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 11657-1-AP

Synonyms

CPSF2L, INT9, integrator complex subunit 9, INTS9, RC 74, RC74

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using INTS9 Polyclonal antibody (11657-1-AP)

    WB analysis of Jurkat using 11657-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using INTS9 Polyclonal antibody (11657-1-AP)

    WB analysis of HeLa using 11657-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using INTS9 Polyclonal antibody (11657-1-AP)

    WB analysis of HeLa using 11657-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using INTS9 Polyclonal antibody (11657-1-AP)

    WB analysis of HEK-293 using 11657-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using INTS9 Polyclonal antibody (11657-1-AP)

    WB analysis of HEK-293 using 11657-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using INTS9 Polyclonal antibody (11657-1-AP)

    WB analysis of Jurkat using 11657-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using INTS9 Polyclonal antibody (11657-1-AP)

    WB analysis of Jurkat using 11657-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using INTS9 Polyclonal antibody (11657-1-AP)

    WB analysis of Jurkat using 11657-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using INTS9 Polyclonal antibody (11657-1-AP)

    WB analysis of Jurkat using 11657-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using INTS9 Polyclonal antibody (11657-1-AP)

    IP experiment of HeLa using 11657-1-AP

    IP result of anti-INTS9 (IP:11657-1-AP, 4ug; Detection:11657-1-AP 1:1000) with HeLa cells lysate 880ug.

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11657-1-AP targets INTS9 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INTS9 fusion protein Ag2226
Full Name integrator complex subunit 9
Calculated Molecular Weight 658 aa, 74 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC025267
Gene Symbol INTS9
Gene ID (NCBI) 55756
RRIDAB_2127514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for INTS9 antibody 11657-1-APDownload protocol
IP protocol for INTS9 antibody 11657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module.

Authors - Kevin Sabath
View Article
humanWB

Protein Expr Purif

CRISPR-Cas9 mediated genetic engineering for the purification of the endogenous integrator complex from mammalian cells.

Authors - David Baillat
View Article
humanWB,IP

Mol Cell

Integrator facilitates RNAPII removal to prevent transcription-replication collisions and genome instability

Authors - Rahul Bhowmick
View Article