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IPO7 Polyclonal antibody
IPO7 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (2)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 28289-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, U-251 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
28289-1-AP targets IPO7 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IPO7 fusion protein Ag27257 |
| Full Name | importin 7 |
| Calculated Molecular Weight | 120 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | NM_006391.2 |
| Gene Symbol | IPO7 |
| Gene ID (NCBI) | 10527 |
| RRID | AB_2881106 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IPO7 antibody 28289-1-AP | Download protocol |
| IHC protocol for IPO7 antibody 28289-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Mechanical control of nuclear import by Importin-7 is regulated by its dominant cargo YAP. | ||
J Cell Biol Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine. | ||
Cells Dev The regulation of Msx1 by BMP4/pSmad1/5 signaling is mediated by importin7 in dental mesenchymal cells.
| ||
iScience Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges | ||
Mol Immunol IPO7 promotes lipopolysaccharide-induced inflammatory responses in human dental pulp cells via p38 MAPK and NF-κB signaling pathways |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH María (Verified Customer) (02-08-2022) | Works fine for WB (1:1000) and IF in permeabilized cells (1:100). Validated with siRNA
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