• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

IRAK3 Polyclonal antibody

IRAK3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28766-1-AP

Synonyms

ASRT5, FLJ13601, IRAK 3, IRAK M, IRAK3, IRAKM

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using IRAK3 Polyclonal antibody (28766-1-AP)

    WB analysis using 28766-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28766-1-AP (IRAK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using IRAK3 Polyclonal antibody (28766-1-AP)

    IHC staining of mouse colon using 28766-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28766-1-AP (IRAK3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using IRAK3 Polyclonal antibody (28766-1-AP)

    IHC staining of rat small intestine using 28766-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28766-1-AP (IRAK3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse spleen tissue, rat spleen tissue
Positive IHC detected inmouse colon tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28766-1-AP targets IRAK3 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRAK3 fusion protein Ag30309
Full Name interleukin-1 receptor-associated kinase 3
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC057800
Gene Symbol IRAK3
Gene ID (NCBI) 11213
RRIDAB_2881211
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IRAK3 antibody 28766-1-APDownload protocol
IHC protocol for IRAK3 antibody 28766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols