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ITK Polyclonal antibody

ITK Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 21525-1-AP

Synonyms

EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ITK Polyclonal antibody (21525-1-AP)

    WB analysis using 21525-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using ITK Polyclonal antibody (21525-1-AP)

    WB analysis of mouse spleen using 21525-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using ITK Polyclonal antibody (21525-1-AP)

    WB analysis of mouse spleen using 21525-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ITK Polyclonal antibody (21525-1-AP)

    WB analysis of Jurkat using 21525-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21525-1-AP (ITK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Jurkat cells using ITK Polyclonal antibody (21525-1-AP)

    FC experiment of Jurkat using 21525-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human ITK (21525-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

Tested Applications

Positive WB detected inJurkat cells, mouse spleen tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

21525-1-AP targets ITK in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ITK fusion protein Ag16132
Full Name IL2-inducible T-cell kinase
Calculated Molecular Weight 620 aa, 72 kDa
Observed Molecular Weight 68-72 kDa
GenBank Accession NumberBC109077
Gene Symbol ITK
Gene ID (NCBI) 3702
RRIDAB_10733884
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ITK antibody 21525-1-APDownload protocol
FC protocol for ITK antibody 21525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cytotherapy

Bruton tyrosine kinase inhibitors preserve anti-CD19 chimeric antigen receptor T-cell functionality and reprogram tumor micro-environment in B-cell lymphoma

Authors - Wenjing Luo
View Article