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Ins1 Monoclonal antibody

Ins1 Monoclonal Antibody for IF, IHC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat

Applications

IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1H2C11

Cat no : 67668-1-Ig

Synonyms

Ins 1, Ins1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Ins1 Monoclonal antibody (67668-1-Ig)

    IHC staining of mouse pancreas using 67668-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67668-1-Ig (Ins1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Ins1 Monoclonal antibody (67668-1-Ig)

    IHC staining of mouse pancreas using 67668-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67668-1-Ig (Ins1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using Ins1 Monoclonal antibody (67668-1-Ig)

    IHC staining of rat pancreas using 67668-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 67668-1-Ig (Ins1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using Ins1 Monoclonal antibody (67668-1-Ig)

    IHC staining of rat pancreas using 67668-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 67668-1-Ig (Ins1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Ins1 Monoclonal antibody (67668-1-Ig)

    IF Staining of mouse pancreas using 67668-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Ins1 antibody (67668-1-Ig, Clone: 1H2C11 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive IHC detected inmouse pancreas tissue, rat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse pancreas tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:3000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67668-1-Ig targets Ins1 in IHC, IF,ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Ins1 fusion protein Ag29988
Full Name insulin I
Calculated Molecular Weight 12 kDa
GenBank Accession NumberNM_008386
Gene Symbol Ins1
Gene ID (NCBI) 16333
RRIDAB_2882864
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

insulin I is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.

Protocols

Product Specific Protocols
IHC protocol for Ins1 antibody 67668-1-IgDownload protocol
IF protocol for Ins1 antibody 67668-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols