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Integrin Beta 4 Monoclonal antibody

Integrin Beta 4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C2A11

Cat no : 66922-2-Ig

Synonyms

CD104, GP150, Integrin beta 4, integrin, beta 4, ITGB4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin Beta 4 Monoclonal antibody (66922-2-Ig)

    WB analysis using 66922-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SW 1990 cells using Integrin Beta 4 Monoclonal antibody (66922-2-Ig)

    WB analysis of SW 1990 using 66922-2-Ig

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Integrin Beta 4 Monoclonal antibody (66922-2-Ig)

    WB analysis using 66922-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using Integrin Beta 4 Monoclonal antibody (66922-2-Ig)

    WB analysis of BxPC-3 using 66922-2-Ig

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin Beta 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using Integrin Beta 4 Monoclonal antibody (66922-2-Ig)

    WB analysis of A549 using 66922-2-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66922-2-Ig (Integrin beta-4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, A549 cells, BxPC-3 cells, SW 1990 cells, SKOV-3 cells, MCF-7 cells, Calu-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66922-2-Ig targets Integrin Beta 4 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Integrin Beta 4 fusion protein Ag17395
Full Name integrin, beta 4
Calculated Molecular Weight 1752 aa, 195 kDa
Observed Molecular Weight 200-210 kDa
GenBank Accession NumberBC126411
Gene Symbol ITGB4
Gene ID (NCBI) 3691
RRIDAB_2882249
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.

Protocols

Product Specific Protocols
WB protocol for Integrin Beta 4 antibody 66922-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols