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  • Featured Product
  • KD/KO Validated

JAKMIP1 Polyclonal antibody

JAKMIP1 Polyclonal Antibody for WB, IP, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13846-1-AP

Synonyms

Gababrbp, JAKMIP1, JAMIP1, Marlin 1, MARLIN1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using JAKMIP1 Polyclonal antibody (13846-1-AP)

    WB analysis of mouse spleen using 13846-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using JAKMIP1 Polyclonal antibody (13846-1-AP)

    WB analysis of Y79 using 13846-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using JAKMIP1 Polyclonal antibody (13846-1-AP)

    WB analysis of HEK-293 using 13846-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using JAKMIP1 Polyclonal antibody (13846-1-AP)

    WB analysis of mouse liver using 13846-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using JAKMIP1 Polyclonal antibody (13846-1-AP)

    WB analysis of mouse testis using 13846-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using JAKMIP1 Polyclonal antibody (13846-1-AP)

    WB analysis of Jurkat using 13846-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13846-1-AP (JAKMIP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using JAKMIP1 Polyclonal antibody (13846-1-AP)

    IP experiment of Jurkat using 13846-1-AP

    IP result of anti-JAKMIP1 (IP:13846-1-AP, 3ug; Detection:13846-1-AP 1:1000) with Jurkat cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using JAKMIP1 Polyclonal antibody (13846-1-AP)

    IHC staining of human gliomas using 13846-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13846-1-AP (JAKMIP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using JAKMIP1 Polyclonal antibody (13846-1-AP)

    IHC staining of human gliomas using 13846-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13846-1-AP (JAKMIP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of Jurkat cells using JAKMIP1 Polyclonal antibody (13846-1-AP)

    FC experiment of Jurkat using 13846-1-AP

    1X10^6 Jurkat cells were stained with 0.2ug JAKMIP1 antibody (13846-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inmouse spleen tissue, HEK-293 cells, Jurkat cells, mouse liver tissue, mouse testis tissue, Y79 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13846-1-AP targets JAKMIP1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JAKMIP1 fusion protein Ag4816
Full Name janus kinase and microtubule interacting protein 1
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC047075
Gene Symbol JAKMIP1
Gene ID (NCBI) 152789
RRIDAB_2128793
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for JAKMIP1 antibody 13846-1-APDownload protocol
IHC protocol for JAKMIP1 antibody 13846-1-APDownload protocol
IP protocol for JAKMIP1 antibody 13846-1-APDownload protocol
FC protocol for JAKMIP1 antibody 13846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Neuron

JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse.

Authors - Jamee M Berg
  • KO Validated
View Article
humanWB,IHC

Biomed Pharmacother

Overexpression of JAKMIP1 associates with Wnt/beta-catenin pathway activation and promotes cancer cell proliferation in vitro.

Authors - Okai Isaac I
View Article