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KCNIP3 Polyclonal antibody

KCNIP3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12032-1-AP

Synonyms

Calsenilin, CSEN, DRE antagonist modulator, DREAM, KCHIP3, KCNIP3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using KCNIP3 Polyclonal antibody (12032-1-AP)

    WB analysis of mouse brain using 12032-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12032-1-AP (KCNIP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using KCNIP3 Polyclonal antibody (12032-1-AP)

    WB analysis of human brain using 12032-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12032-1-AP (KCNIP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using KCNIP3 Polyclonal antibody (12032-1-AP)

    WB analysis of mouse brain using 12032-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12032-1-AP (KCNIP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KCNIP3 Polyclonal antibody (12032-1-AP)

    IP experiment of mouse brain using 12032-1-AP

    IP result of anti-KCNIP3 (IP:12032-1-AP, 4ug; Detection:12032-1-AP 1:1000) with mouse brain tissue lysate 4400 ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using KCNIP3 Polyclonal antibody (12032-1-AP)

    IHC staining of human gliomas using 12032-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12032-1-AP (KCNIP3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using KCNIP3 Polyclonal antibody (12032-1-AP)

    IHC staining of human gliomas using 12032-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12032-1-AP (KCNIP3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using KCNIP3 Polyclonal antibody (12032-1-AP)

    IF Staining of SH-SY5Y using 12032-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 12032-1-AP (KCNIP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12032-1-AP targets KCNIP3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNIP3 fusion protein Ag2665
Full Name Kv channel interacting protein 3, calsenilin
Calculated Molecular Weight 256 aa, 29 kDa
Observed Molecular Weight 29 kDa, 27 kDa
GenBank Accession NumberBC012850
Gene Symbol KCNIP3
Gene ID (NCBI) 30818
RRIDAB_2234348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KCNIP3 antibody 12032-1-APDownload protocol
IHC protocol for KCNIP3 antibody 12032-1-APDownload protocol
IF protocol for KCNIP3 antibody 12032-1-APDownload protocol
IP protocol for KCNIP3 antibody 12032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols