• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RBP2 Polyclonal antibody

RBP2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 18825-1-AP

Synonyms

Histone demethylase JARID1A, JARID1A, KDM5A, lysine (K) demethylase 5A, Lysine specific demethylase 5A, RBBP 2, RBBP2, RBP2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using RBP2 Polyclonal antibody (18825-1-AP)

    WB analysis of HeLa using 18825-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18825-1-AP (RBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RBP2 Polyclonal antibody (18825-1-AP)

    IP experiment of HeLa using 18825-1-AP

    IP result of anti-RBP2 (IP:18825-1-AP, 4ug; Detection:18825-1-AP 1:500) with HeLa cells lysate 1280 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBP2 Polyclonal antibody (18825-1-AP)

    IF Staining of HeLa using 18825-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18825-1-AP (RBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using RBP2 Polyclonal antibody (18825-1-AP)

    IF Staining of mouse small intestine using 18825-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using RBP2 Polyclonal antibody (18825-1-AP)

    IF Staining of mouse small intestine using 18825-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells, mouse small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18825-1-AP targets RBP2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name lysine (K)-specific demethylase 5A
Calculated Molecular Weight 192 kDa
Observed Molecular Weight 192 kDa
GenBank Accession NumberNM_005056
Gene Symbol KDM5A
Gene ID (NCBI) 5927
RRIDAB_10638436
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KDM5A, also named as JARID1A, RBBP2 and RBP2, belongs to the JARID1 histone demethylase family. KDM5A is a Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. RBP2 may stimulate transcription mediated by nuclear receptors. It may be involved in transcriptional regulation of Hox proteins during cell differentiation. It may participate in transcriptional repression of cytokines such as CXCL12.

Protocols

Product Specific Protocols
WB protocol for RBP2 antibody 18825-1-APDownload protocol
IF protocol for RBP2 antibody 18825-1-APDownload protocol
IP protocol for RBP2 antibody 18825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Environ Toxicol

JMJD3 downregulates IL4i1 aggravating lipopolysaccharide-induced acute lung injury via H3K27 and H3K4 demethylation

Authors - Yizhuo Gao
View Article
humanWB

Int J Biol Sci

KDM5B demethylates H3K4 to recruit XRCC1 and promote chemoresistance.

Authors - Wenxia Xu
View Article
ratIF

Life Sci

Treadmill exercise improves neurological function by inhibiting autophagy and the binding of HMGB1 to Beclin1 in MCAO juvenile rats.

Authors - Guoyuan Pan
View Article