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KIAA0907 Polyclonal antibody

KIAA0907 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25419-1-AP

Synonyms

BLOM7, KHDC4, KIAA0907, RP11 336K24.1, UPF0469 protein KIAA0907

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA0907 Polyclonal antibody (25419-1-AP)

    WB analysis using 25419-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25419-1-AP (KIAA0907 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KIAA0907 Polyclonal antibody (25419-1-AP)

    IHC staining of human lung cancer using 25419-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25419-1-AP (KIAA0907 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using KIAA0907 Polyclonal antibody (25419-1-AP)

    IHC staining of human lung cancer using 25419-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25419-1-AP (KIAA0907 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, PC-13 cells, mouse liver tissue, mouse small intestine tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25419-1-AP targets KIAA0907 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA0907 fusion protein Ag22217
Full Name KIAA0907
Calculated Molecular Weight 614 aa, 65 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC062637
Gene Symbol KIAA0907
Gene ID (NCBI) 22889
RRIDAB_2880070
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA0907, also named as BLOM7, is 614 amino acid protein, which belongs to the UPF0469 family. KIAA0907 exists as four alternative splicing isoforms and its residues is modified by phosphorylase. KIAA0907 are frequently observed in lung cancer cells.

Protocols

Product Specific Protocols
WB protocol for KIAA0907 antibody 25419-1-APDownload protocol
IHC protocol for KIAA0907 antibody 25419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols