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KIAA1984 Polyclonal antibody

KIAA1984 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20945-1-AP

Synonyms

KIAA1984, PARF, RP11 216L13.9

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KIAA1984 Polyclonal antibody (20945-1-AP)

    WB analysis of HeLa using 20945-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20945-1-AP (KIAA1984 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using KIAA1984 Polyclonal antibody (20945-1-AP)

    WB analysis of HepG2 using 20945-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20945-1-AP (KIAA1984 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KIAA1984 Polyclonal antibody (20945-1-AP)

    IF Staining of HepG2 using 20945-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20945-1-AP (KIAA1984 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20945-1-AP is KIAA1984 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIAA1984 fusion protein Ag15053
Full Name KIAA1984
Calculated Molecular Weight 534 aa, 63 kDa
Observed Molecular Weight 45-55 kDa
GenBank Accession NumberBC007542
Gene Symbol KIAA1984
Gene ID (NCBI) 84960
RRIDAB_2878772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KIAA1984 antibody 20945-1-APDownload protocol
IF protocol for KIAA1984 antibody 20945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols