KIFAP3 Monoclonal antibody

KIFAP3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

7C9F2

Cat no : 60266-1-Ig

Synonyms

KAP 3, KAP3, KIF3AP, KIFAP3, kinesin associated protein 3, SMAP, Smg GDS, Smg GDS associated protein



Tested Applications

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 60266-1-Ig is KIFAP3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen KIFAP3 fusion protein Ag20809
Full Name kinesin-associated protein 3
Calculated Molecular Weight 792 aa, 91 kDa
Observed Molecular Weight 91-100 kDa
GenBank Accession NumberBC028679
Gene Symbol KIFAP3
Gene ID (NCBI) 22920
RRIDAB_2881387
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIFAP3, also known as KIF3AP or KAP3, is a novel KIF3A/3B-associated protein. It binds to the tail domain of KIF3A/3B and may play a role in regulating the binding of KIF3A/3B to cargoes. Recently it has been reported that mutation within the KIFAP3 gene is associated with decreased KIFAP3 expression and increased survival in sporadic ALS, which makes it a potential target for ALS therapy. KIFAP3 has also been identified as the target of mir-130a.

Protocols

Product Specific Protocols
WB protocol for KIFAP3 antibody 60266-1-IgDownload protocol
IHC protocol for KIFAP3 antibody 60266-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols