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KRT12 Polyclonal antibody

KRT12 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24789-1-AP

Synonyms

CK 12, Cytokeratin 12, K12, keratin 12, KRT12

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse eye tissue using KRT12 Polyclonal antibody (24789-1-AP)

    WB analysis of mouse eye using 24789-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 24789-1-AP (KRT12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using KRT12 Polyclonal antibody (24789-1-AP)

    IHC staining of mouse eye using 24789-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 24789-1-AP (KRT12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse eye tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 6 publications below

Product Information

24789-1-AP targets KRT12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KRT12 fusion protein Ag20312
Full Name keratin 12
Calculated Molecular Weight 494 aa, 54 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC156641
Gene Symbol KRT12
Gene ID (NCBI) 3859
RRIDAB_2879726
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KRT12 antibody 24789-1-APDownload protocol
IHC protocol for KRT12 antibody 24789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

EBioMedicine

Cisd2 plays an essential role in corneal epithelial regeneration.

Authors - Chi-Chin Sun
View Article
mouseIF

Invest Ophthalmol Vis Sci

Wounding Induces Facultative Opn5-Dependent Circadian Photoreception in the Murine Cornea.

Authors - Nicolás M Díaz
View Article
mouseIF

Exp Eye Res

Low-dose repeated exposure to chemical surfactant impairs corneal epithelium: When personal cleaning products entering the eye.

Authors - Jie Wu
View Article
humanIF

Int Ophthalmol

ZD6474 attenuates TGF-β1-induced fibrosis in human Tenon fibroblasts and inhibits neovascularization via AKT-mTOR signaling pathway

Authors - Wenting Liu
View Article
humanIF

J Ocul Pharmacol Ther

ZD6474 Attenuates Fibrosis and Inhibits Neovascularization in Human Pterygium by Suppressing AKT-mTOR Signaling Pathway

Authors - Wenting Liu
View Article
mouseIF

Transl Vis Sci Technol

Unraveling the Intricate Network of lncRNAs in Corneal Epithelial Wound Healing: Insights Into the Regulatory Role of linc17500

Authors - Qiongjie Cao
View Article