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LARP4 Polyclonal antibody

LARP4 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16529-1-AP

Synonyms

La related protein 4, LARP4, PP13296

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using LARP4 Polyclonal antibody (16529-1-AP)

    WB analysis of Y79 using 16529-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 16529-1-AP (LARP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using LARP4 Polyclonal antibody (16529-1-AP)

    WB analysis of Y79 using 16529-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 16529-1-AP (LARP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Y79 cells using LARP4 Polyclonal antibody (16529-1-AP)

    IP experiment of Y79 using 16529-1-AP

    IP result of anti-LARP4 (IP:16529-1-AP, 4ug; Detection:16529-1-AP 1:300) with Y79 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using LARP4 Polyclonal antibody (16529-1-AP)

    IHC staining of human brain using 16529-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using LARP4 Polyclonal antibody (16529-1-AP)

    IHC staining of human brain using 16529-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using LARP4 Polyclonal antibody (16529-1-AP)

    IHC staining of human spleen using 16529-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using LARP4 Polyclonal antibody (16529-1-AP)

    IHC staining of human spleen using 16529-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using LARP4 Polyclonal antibody (16529-1-AP)

    IHC staining of human lung using 16529-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using LARP4 Polyclonal antibody (16529-1-AP)

    IHC staining of human lung using 16529-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using LARP4 Polyclonal antibody (16529-1-AP)

    IHC staining of human ovary using 16529-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using LARP4 Polyclonal antibody (16529-1-AP)

    IHC staining of human ovary using 16529-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LARP4 Polyclonal antibody (16529-1-AP)

    IHC staining of human kidney using 16529-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using LARP4 Polyclonal antibody (16529-1-AP)

    IHC staining of human kidney using 16529-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LARP4 Polyclonal antibody (16529-1-AP)

    IHC staining of human testis using 16529-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using LARP4 Polyclonal antibody (16529-1-AP)

    IHC staining of human testis using 16529-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16529-1-AP (LARP4 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inY79 cells
Positive IP detected inY79 cells
Positive IHC detected inhuman brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16529-1-AP targets LARP4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LARP4 fusion protein Ag9777
Full Name La ribonucleoprotein domain family, member 4
Calculated Molecular Weight 188 aa, 21 kDa, 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC022377
Gene Symbol LARP4
Gene ID (NCBI) 113251
RRIDAB_2296671
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LARP4 antibody 16529-1-APDownload protocol
IHC protocol for LARP4 antibody 16529-1-APDownload protocol
IP protocol for LARP4 antibody 16529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,IP

Front Cell Dev Biol

Interaction of LARP4 to filamin A mechanosensing domain regulates cell migrations

Authors - Zhenfeng Mao
View Article