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LPO Polyclonal antibody

LPO Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10376-1-AP

Synonyms

lactoperoxidase, LPO, Salivary peroxidase, SAPX, SPO

Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using LPO Polyclonal antibody (10376-1-AP)

    WB analysis of human saliva using 10376-1-AP

    human saliva were subjected to SDS PAGE followed by western blot with 10376-1-AP (LPO Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of K-562 cells using LPO Polyclonal antibody (10376-1-AP)

    WB analysis of K-562 using 10376-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10376-1-AP (LPO Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LPO Polyclonal antibody (10376-1-AP)

    IHC staining of human colon cancer using 10376-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10376-1-AP (LPO Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using LPO Polyclonal antibody (10376-1-AP)

    IHC staining of human colon cancer using 10376-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10376-1-AP (LPO Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LPO Polyclonal antibody (10376-1-AP)

    IHC staining of human breast cancer using 10376-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10376-1-AP (LPO Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman saliva tissue, K-562 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10376-1-AP targets LPO in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen peptide
Full Name lactoperoxidase
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC107167
Gene Symbol LPO
Gene ID (NCBI) 4025
RRIDAB_2877734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LPO antibody 10376-1-APDownload protocol
IHC protocol for LPO antibody 10376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Biochemical properties of thyroid peroxidase (TPO) expressed in human breast and mammary-derived cell lines.

Authors - Marlena Godlewska
View Article

PLoS One

Thyroid peroxidase (TPO) expressed in thyroid and breast tissues shows similar antigenic properties.

Authors - Marlena Godlewska
View Article
humanIHC

Front Cell Dev Biol

Identification and verification of a prognostic signature based on a miRNA-mRNA interaction pattern in colon adenocarcinoma

Authors - Qiwu Zhao
View Article