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LPPR2 Polyclonal antibody

LPPR2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25732-1-AP

Synonyms

LPPR2, PRG 4, PRG4

Validation Data Gallery

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  • Western Blot (WB) analysis of 3T3-L1 cells using LPPR2 Polyclonal antibody (25732-1-AP)

    WB analysis of 3T3-L1 using 25732-1-AP

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 25732-1-AP (LPPR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LPPR2 Polyclonal antibody (25732-1-AP)

    WB analysis of HeLa using 25732-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25732-1-AP (LPPR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using LPPR2 Polyclonal antibody (25732-1-AP)

    IHC staining of human skeletal muscle using 25732-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25732-1-AP (LPPR2 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using LPPR2 Polyclonal antibody (25732-1-AP)

    IHC staining of human skeletal muscle using 25732-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25732-1-AP (LPPR2 Antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected in3T3-L1 cells, HeLa cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25732-1-AP is LPPR2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LPPR2 fusion protein Ag22839
Full Name lipid phosphate phosphatase-related protein type 2
Calculated Molecular Weight 427 aa, 46 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC009378
Gene Symbol LPPR2
Gene ID (NCBI) 64748
RRIDAB_2880215
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LPPR2 antibody 25732-1-APDownload protocol
IHC protocol for LPPR2 antibody 25732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Placenta

SWATH proteomics analysis of placental tissue with intrahepatic cholestasis of pregnancy

Authors - Yuxuan Jiang
View Article