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KD/KO Validated

PTPRA Polyclonal antibody

Name: PTPRA Polyclonal antibody
Brand: Proteintech
SKU: 13079-1-AP

PTPRA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13079-1-AP

Synonyms

HEPTP, HLPR, HPTPA, HPTPalpha, LRP, PTPA, PTPRA, PTPRL2, R PTP alpha, RPTPA

Validation Data Gallery

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AllWBIPIHCIF

WB analysis of rat liver using 13079-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 13079-1-AP (PTPRA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis using 13079-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13079-1-AP (PTPRA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 13079-1-AP
IP result of anti-PTPRA (IP:13079-1-AP, 4ug; Detection:13079-1-AP 1:300) with A431 cells lysate 2400ug.
IHC staining of rat heart using 13079-1-AP
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 13079-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13079-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 13079-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 13079-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 13079-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13079-1-AP (PTPRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 13079-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using PTPRA antibody (13079-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 13079-1-AP

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Tested Applications

Positive WB detected in	A431 cells, rat liver tissue, MCF-7 cells, SKOV-3 cells
Positive IP detected in	A431 cells
Positive IHC detected in	human lung cancer tissue, human urothelial carcinoma tissue, mouse heart tissue, mouse kidney tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

13079-1-AP targets PTPRA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity	human, rat, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PTPRA fusion protein Ag3760
Full Name	protein tyrosine phosphatase, receptor type, A
Calculated Molecular Weight	793 aa, 91 kDa
Observed Molecular Weight	90-105 kDa, 140 kDa
GenBank Accession Number	BC027308
Gene Symbol	PTPRA
Gene ID (NCBI)	5786
RRID	AB_2269055
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PTPRA antibody 13079-1-AP	Download protocol
IHC protocol for PTPRA antibody 13079-1-AP	Download protocol
IF protocol for PTPRA antibody 13079-1-AP	Download protocol
IP protocol for PTPRA antibody 13079-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncol Lett PTPRA facilitates cancer growth and migration via the TNF-α-mediated PTPRA-NF-κB pathway in MCF-7 breast cancer cells. Authors - Canfeng Lin KO Validated View Article
human	WB,IF,IP	Oncogene Glucose-mediated N-glycosylation of RPTPα affects its subcellular localization and Src activation Authors - Jiayu Fang View Article
mouse	WB	Sci Signal Clustering of phosphatase RPTPα promotes Src signaling and the arthritogenic action of synovial fibroblasts Authors - Sho Sendo View Article
human	WB	EBioMedicine The circRNA circPTPRA suppresses epithelial-mesenchymal transitioning and metastasis of NSCLC cells by sponging miR-96-5p. Authors - Siliang Wei KD Validated View Article

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PTPRA Polyclonal antibody

PTPRA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of rat liver using 13079-1-AP

WB analysis using 13079-1-AP

IP experiment of A431 using 13079-1-AP

IHC staining of rat heart using 13079-1-AP

IHC staining of rat kidney using 13079-1-AP

IHC staining of human lung cancer using 13079-1-AP

IHC staining of human urothelial carcinoma using 13079-1-AP

IHC staining of mouse heart using 13079-1-AP

IHC staining of mouse kidney using 13079-1-AP

IF Staining of A431 using 13079-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PTPRA facilitates cancer growth and migration via the TNF-α-mediated PTPRA-NF-κB pathway in MCF-7 breast cancer cells.

Glucose-mediated N-glycosylation of RPTPα affects its subcellular localization and Src activation

Clustering of phosphatase RPTPα promotes Src signaling and the arthritogenic action of synovial fibroblasts

The circRNA circPTPRA suppresses epithelial-mesenchymal transitioning and metastasis of NSCLC cells by sponging miR-96-5p.