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LSP1 Polyclonal antibody

LSP1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15593-1-AP

Synonyms

47 kDa actin binding protein, 52 kDa phosphoprotein, LSP1, lymphocyte protein 1, Lymphocyte specific protein 1, pp52, WP34

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using LSP1 Polyclonal antibody (15593-1-AP)

    WB analysis of Raji using 15593-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 15593-1-AP (LSP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using LSP1 Polyclonal antibody (15593-1-AP)

    WB analysis using 15593-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15593-1-AP (LSP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using LSP1 Polyclonal antibody (15593-1-AP)

    WB analysis of U-937 using 15593-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 15593-1-AP (LSP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using LSP1 Polyclonal antibody (15593-1-AP)

    IP experiment of Raji using 15593-1-AP

    IP result of anti-LSP1 (IP:15593-1-AP, 4ug; Detection:15593-1-AP 1:300) with Raji cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using LSP1 Polyclonal antibody (15593-1-AP)

    IHC staining of human colon using 15593-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15593-1-AP (LSP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHL-60 cells, U-937 cells, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15593-1-AP targets LSP1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LSP1 fusion protein Ag7944
Full Name lymphocyte-specific protein 1
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC001785
Gene Symbol LSP1
Gene ID (NCBI) 4046
RRIDAB_2250134
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LSP1 antibody 15593-1-APDownload protocol
IHC protocol for LSP1 antibody 15593-1-APDownload protocol
IP protocol for LSP1 antibody 15593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols