Luciferase Polyclonal antibody
Luciferase Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
firefly, recombinant protein and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 27986-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
Product Information
27986-1-AP targets Luciferase in WB, ELISA applications and shows reactivity with firefly, recombinant protein samples.
| Tested Reactivity | firefly, recombinant protein |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Luciferase fusion protein Ag27633 |
| Full Name | Luciferase |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881031 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Luciferase produces green light with the wavelength of 562 nm. Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light. This antibody detects the Luciferase protein of Photinus pyralis (Common eastern firefly) (Lampyris pyralis).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Luciferase antibody 27986-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics. | ||
EMBO J A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression. | ||
Nat Commun A plant RNA virus inhibits NPR1 sumoylation and subverts NPR1-mediated plant immunity | ||
J Virol SARS-CoV-2 nsp13 Restricts Episomal DNA Transcription without Affecting Chromosomal DNA |
