FITC Plus Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5)
Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2b, kappa
Reactivity
Mouse and More (1)
Applications
FC
Conjugate
FITC Plus Fluorescent Dye
CloneNo.
RB6-8C5
Cat no : FITC-65140
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | mouse bone marrow cells |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| FC | See 2 publications below |
Product Information
FITC-65140 targets Ly-6G/Ly-6C (Gr-1) in FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Raised against granulocytes of mouse origin |
| Full Name | lymphocyte antigen 6 complex, locus G |
| GenBank Accession Number | X70920 |
| Gene Symbol | Ly6g |
| Gene ID (NCBI) | 546644 |
| RRID | AB_2883800 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus Ly-6G/Ly-6C (Gr-1) antibody FITC-65140 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Cancer-Associated Fibroblast-Induced Remodeling of Tumor Microenvironment in Recurrent Bladder Cancer | ||
Front Immunol Endogenous α7 nAChR Agonist SLURP1 Facilitates Escherichia coli K1 Crossing the Blood-Brain Barrier. |
