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MAPKSP1 Polyclonal antibody

MAPKSP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11937-1-AP

Synonyms

LAMTOR3, MAP2K1IP1, MAPBP, MAPK scaffold protein 1, MAPKSP1, MEK binding partner 1, MP1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human liver tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    WB analysis of human liver using 11937-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11937-1-AP (MAPKSP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    WB analysis of mouse brain using 11937-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11937-1-AP (MAPKSP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    WB analysis of mouse liver using 11937-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11937-1-AP (MAPKSP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using MAPKSP1 Polyclonal antibody (11937-1-AP)

    WB analysis of L02 using 11937-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 11937-1-AP (MAPKSP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    IHC staining of human prostate cancer using 11937-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    IHC staining of human heart using 11937-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    IHC staining of human heart using 11937-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    IHC staining of human liver using 11937-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    IHC staining of human liver using 11937-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    IHC staining of human prostate cancer using 11937-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MAPKSP1 Polyclonal antibody (11937-1-AP)

    IHC staining of human prostate cancer using 11937-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11937-1-AP (MAPKSP1 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman liver tissue, L02 cells, mouse brain tissue, mouse liver tissue
Positive IHC detected inhuman prostate cancer tissue, human heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11937-1-AP targets MAPKSP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAPKSP1 fusion protein Ag2534
Full Name MAPK scaffold protein 1
Calculated Molecular Weight 124 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC026245
Gene Symbol MAPKSP1
Gene ID (NCBI) 8649
RRIDAB_2141610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAPKSP1, also named as mitogen activated protein kinase kinase 1 interacting protein 1, Map2k1ip1, MEK partner 1, Lamtor3 and MP1. It was first identified as a scaffold protein enhancing the efficiency of the MAPK pathway by facilitating the interaction between MEK1 and ERK1 upon serum stimulation (PMID: 9733512). Later, it has been demonstrated that MAPKSP1 enhances activation of both ERK1 and ERK2 as a response to EGF (PMID: 12479806) and fibronectin (PMID: 15923628). MAPKSP1 is involved in regulating endosomal signaling (PMID: 17178906), and a role in cancer, via MEK and ERK hyperactivation, has been demostrated in pancreatic tumorigenesis (PMID: 24209743). MAPKSP1 has two isoforms with the molecular weight of 13-14 kDa.

Protocols

Product Specific Protocols
WB protocol for MAPKSP1 antibody 11937-1-APDownload protocol
IHC protocol for MAPKSP1 antibody 11937-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Pharmacol

Brucea javanica Oil Emulsion Promotes Autophagy in Ovarian Cancer Cells Through the miR-8485/LAMTOR3/mTOR/ATG13 Signaling Axis

Authors - Yihan Wang
View Article